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(A) Cortical lysate from P60 control and γC5 KO were probed with synaptic proteins <t>(PSD95,</t> gephyrin, and synapsin) via Western blotting. (B) No significant differences were observed between control and γC5 KO. n = 8 mice/genotype, run in duplicate. Each dot represents one mouse averaged from at least 2 separate Western blot experiments. Unpaired t-test; error bars represent the SEM; ns = not significant.
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(A) Cortical lysate from P60 control and γC5 KO were probed with synaptic proteins (PSD95, gephyrin, and synapsin) via Western blotting. (B) No significant differences were observed between control and γC5 KO. n = 8 mice/genotype, run in duplicate. Each dot represents one mouse averaged from at least 2 separate Western blot experiments. Unpaired t-test; error bars represent the SEM; ns = not significant.

Journal: PLOS One

Article Title: A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex

doi: 10.1371/journal.pone.0344863

Figure Lengend Snippet: (A) Cortical lysate from P60 control and γC5 KO were probed with synaptic proteins (PSD95, gephyrin, and synapsin) via Western blotting. (B) No significant differences were observed between control and γC5 KO. n = 8 mice/genotype, run in duplicate. Each dot represents one mouse averaged from at least 2 separate Western blot experiments. Unpaired t-test; error bars represent the SEM; ns = not significant.

Article Snippet: Pan-γ-Pcdh N159/5 (Mouse, 1:500, NeuroMab 75−185, RRID:AB_2877195); Pan-γ-Pcdh-A N144/32 (Mouse, 1:500, NeuroMab 75−178 RRID:AB_2877459); γ-Pcdh-B2 N148/30 (Mouse, 1:500, NeuroMab 75−184, RRID:AB_2877457); γ-Pcdh-C5 (Mouse, 1:500, Invitrogen MA5−26922 RRID:AB_2724958); GAPDH (Mouse, 1:1000, Abcam 8245, RRID:AB_2107448); CC3 (Rabbit, 1:100, Cell Signaling 9661, RRID:AB_2341188); GFAP (Mouse, 1:500, Sigma-Aldrich G3893, RRID:AB_477010); NeuN (Mouse, 1:300, Millipore MAB377 RRID:AB_2298772); Cux1 (Rabbit, 1:100, Proteintech 11733–1-AP RRID:AB_2086995); Foxp2 (Rabbit, 1:500, Abcam 16046 RRID:AB_2107107); PSD95 (Mouse, 1:1000, Cell Signaling 36233 RRID:AB_2721262); Gephyrin (Rabbit, 1:1000, Cell Signaling 14304, RRID:AB_2798443); Synapsin (Rabbit, 1:1000, Cell Signaling 5297 RRID:AB_2616578).

Techniques: Control, Western Blot